Table 3.
MFAT and LP secretome at 3, 7, 14, and 28 days in vitro.
Analytes | 3 days of culture | 7 days of culture | 14 days of culture | 28 days of culture | ||||
---|---|---|---|---|---|---|---|---|
MFAT-CM | LP-CM | MFAT-CM | LP-CM | MFAT-CM | LP-CM | MFAT-CM | LP-CM | |
IL-1rα | 55 ± 18 | 53 ± 17 | 51 ± 27 | 151 ± 78 | 121 ± 43 | — | 48 ± 23 | — |
IL-6 | 65 ± 23 | 276 ± 67 | 403 ± 98 | 3982 ± 90∗ | 219 ± 87∗ | 7 ± 5 | 95 ± 31 | 3 ± 2 |
IL-8 | 3770 ± 1245 | 2860 ± 890 | 4777 ± 1769 | 8108 ± 1422∗ | 1038 ± 432 | 512 ± 102 | 463 ± 145∗ | 112 ± 46 |
IL-12p70 | — | — | — | 220 ± 88 | 114 ± 32 | — | 77 ± 14 | — |
β-FGF | 650 ± 231 | 518 ± 198 | 302 ± 96 | 134 ± 67 | 130 ± 54 | 154 ± 56 | 66 ± 25 | 126 ± 85 |
G-CSF | 398 ± 123 | 839 ± 234 | 73 ± 54 | 238 ± 98 | 180 ± 38 | — | — | — |
GM-CSF | 79 ± 32 | 61 ± 34 | 61 ± 43 | 73 ± 13 | 62 ± 25 | 71 ± 34 | 60 ± 35 | — |
MCP-1 | 317 ± 145 | 317 ± 78 | 283 ± 98 | 383 ± 121 | 351 ± 87∗ | — | 249 ± 124∗ | |
PDGF-BB | 47 ± 239 | 32 ± 16 | — | — | — | — | — | — |
RANTES | 193 ± 87 | 49 ± 21 | — | 114 ± 33 | — | — | — | — |
VEGF | 515 ± 145 | 251 ± 78 | 308 ± 30 | 1446 ± 799∗ | 749 ± 68∗ | 123 ± 87 | 479±39∗∗ | 63 ± 18 |
IL-2rα | — | — | — | — | 52 ± 18 | — | 47 ± 26 | — |
IL-3 | 82 ± 43 | 66 ± 31 | 62 ± 15 | 81 ± 34 | 89 ± 25 | — | 75 ± 27− | |
IL-12p40 | 224 ± 56 | 202 ± 50 | 170 ± 32 | 191 ± 87 | 202 ± 46 | 70 ± 31 | 201 ± 86∗ | — |
IL-16 | 332 ± 89 | 124 ± 43 | 130 ± 56 | 213 ± 111 | 58 ± 57 | — | 47 ± 15 | — |
GROa | 68 ± 22 | 102 ± 42 | 134 ± 87 | 787 ± 234 | 58 ± 32 | 30 ± 15 | 41 ± 18 | — |
HGF | 12003 ± 4657 | 7315 ± 2354 | 5490 ± 1677 | 5584 ± 1989 | 13442±3687∗∗ | 772 ± 232 | 9839±2341∗∗ | 239 ± 97 |
LIF | 56 ± 16 | 56 ± 17 | 61 ± 23 | 177 ± 89 | 32 ± 13 | — | 17 ± 3 | — |
M-CSF | 252 ± 69 | 116 ± 43 | 271 ± 99 | 354 ± 143 | 124 ± 65 | — | 60 ± 9 | — |
MIF | 7912 ± 2368 | 10086 ± 2899 | 4565 ± 908 | 3382 ± 970 | 7915±768∗∗ | 1407 ± 398 | 6430±11387∗∗ | 127 ± 54 |
MIG | 925 ± 329 | 1323 ± 675 | 1390 ± 455 | 4048 ± 1324 | 195 ± 76∗ | — | 51 ± 24 | — |
SCF | 71 ± 45 | 114 ± 65 | 166 ± 77 | 205 ± 54 | 41 ± 32 | — | 22 ± 9 | — |
SCGF-β | 5591 ± 1457 | 1343 ± 630 | 1203 ± 544 | 2492 ± 545 | 5699±1875∗∗ | 201 ± 54 | 4560±799∗∗ | — |
SDF-1 | 72 ± 21 | 82 ± 29 | 71 ± 32 | 96 ± 29 | 106 ± 51 | 62 ± 32 | 99 ± 30 | — |
TRAIL | 85 ± 47 | 46 ± 12 | 37 ± 11 | 57 ± 21 | 41 ± 10 | — | — | — |
Total | 33, 759 ± 6567 | 26, 279 ± 5688 | 20, 039 ± 4387 | 32, 872 ± 9854∗ | 31, 059±8975∗∗ | 3, 329 ± 1254 | 23, 057±6590∗∗ | 706 ± 154 |
∗ p < 0.05; ∗∗p < 0.01 MFAT-CM vs. LP-CM.