TABLE 3.

Additivity of ΔLog(d) values for M2 and M3 mutations by drug

Values for wild type are log(d) ± S.D. from fits of eq. 3 (Materials and Methods) to estimated P_open data (see Fig. 4; Table 2). Values for mutant receptors represent differences between log(d) values for wild type and mutants.

Mutation	ΔLog(d) (Mutant − Wild Type) ± S.D.
	Etomidate	mTFD-MPAB	Propofol	Propofol (Adjusted)a
Wild type	−1.91 ± 0.075	−2.13 ± 0.065	−1.89 ± 0.065	−1.89 ± 0.065
ΜM2-15′
α1S270I	0.33 ± 0.086*	1.71 ± 0.074	1.45 ± 0.076	1.12 ± 0.11
β3N265M	1.89 ± 0.078	0.64 ± 0.10*	1.81 ± 0.069	1.17 ± 0.12
γ2S280W	0.70 ± 0.086*	2.29 ± 0.077	1.50 ± 0.075	0.80 ± 0.11
Sum (x − 1)	−2.9 ± 0.15	−4.6 ± 0.15	−4.8 ± 0.13	−3.1 ± 0.20
ΜM3-36′
α1A291W	0.69 ± 0.084*	1.6 ± 0.96	0.96 ± 0.085	0.27 ± 0.12
β3M286W	1.7 ± 0.12	0.93 ± 0.079*	1.7 ± 0.11	0.76 ± 0.13
γ2S301W	1.19 ± 0.092*	1.88 ± 0.084	1.25 ± 0.091	0.06 ± 0.13
Sum (x − 1)	−3.6 ± 0.17	−4.5 ± 0.15	−3.9 ± 0.16	−1.1 ± 0.22

^aAdjusted propofol Δlog(d) values were calculated by subtracting presumed allosteric mutant effects based on etomidate and R-mTFD-MPAB experiments (identified by an asterisk in each mutant row) from unadjusted propofol Δlog(d) values.