Released M.tb RNA in the culture supernatant from the same number of mycobacteria.
RNA bioanalyzer analysis for total RNA isolated from EVs released by WT, ΔsecA2, or secA2‐complemented (ΔsecA2_C) M.tb strains.
Quantification of EV total RNA analyzed in (B).
Quantification of total proteins of EVs from various M.tb strain‐infected BMMs.
Western blot analysis for host and M.tb proteins in EVs.
M.tb survival assay in wild‐type mouse BMMs.
Data information: Data shown in (A, C, D and F) are the mean ± SD of three independent cultures (A) or infections (C, D and F), and all results shown are representative of three independent experiments. **
P < 0.01 by Student's
t‐test (two‐tailed).
Source data are available online for this figure.