Skip to main content
. 2019 Jan 8;20(3):e47468. doi: 10.15252/embr.201847468

Figure 4. Myoparr interacts with DEAD box protein Ddx17.

Figure 4

  • A
    Scheme of the identification of Myoparr‐interacting proteins using RiboTrap and differential proteomics analysis.
  • B
    Following immunoprecipitation, the interaction between Myoparr and endogenous Ddx17 was confirmed by immunoblotting using a Ddx17‐specific antibody. Two different Ddx17 isoforms (p72 and p82) were observed.
  • C
    Determination of the Ddx17‐binding region of Myoparr by RNA pull‐down analyses. Schematic diagram of full‐length or truncated Myoparr used for RNA pull‐down (top). In vitro‐transcribed/in vitro‐translated Ddx17 protein was pulled down by indicated Myoparr and then detected by Western blot using a Ddx17 antibody (bottom).
  • D
    Schematic diagram of the constructs used for luciferase assays. The details of all constructs are described in Materials and Methods. p(A) indicates poly(A) site.
  • E–G
    Relative luciferase activities of indicated constructs in differentiating C2C12 cells.
  • H
    Relative luciferase activities of indicated constructs in differentiating C2C12 cells transfected with control or Myoparr shRNA (Myoparr KD).
Data information: In (E‐H), bars indicate the average of two independent experiments, and open circles represent the values of each experiment.