Skip to main content
. 2019 Jan 8;20(3):e47468. doi: 10.15252/embr.201847468

Figure 6. Myoparr and Ddx17 regulate myoblast cell cycle withdrawal in a myogenin‐independent manner.

Figure 6

  • A
    Heatmap displaying expression changes of 754 genes significantly altered either in Myoparr‐ or Ddx17‐depleted cells (log 2 ratio scale).
  • B
    The intersection of genes regulated by Myoparr KD and Ddx17 KD shows a significant (Fisher's exact test) overlap 26.9 times as large as that expected by chance alone.
  • C
    Genes significantly differentially expressed in both Myoparr‐ and Ddx17‐depleted cells show correlated expression (R = 0.94, log 2 ratio scale).
  • D–F
    Enrichment analysis of GO categories in genes up‐regulated by Ddx17 KD (D), down‐regulated by Ddx17 KD (E), and commonly regulated by both Myoparr KD and Ddx17 KD (F).
  • G
    Myoparr and Ddx17 are required for C2C12 cell cycle withdrawal. C2C12 cells transfected with each siRNA were cultured in growth medium for 24 h. After differentiation induction, cells were maintained in differentiation medium for 40 h and then treated with EdU for 6 h. EdU‐positive cells are shown as percent of the control. Nuclei were counterstained with Hoechst 33342. n = 3, mean ± SD. **P < 0.01 (unpaired two‐tailed Student's t‐test). Scale bar, 100 μm.