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. 2019 Jan 8;20(3):e47468. doi: 10.15252/embr.201847468

Figure 7. Myoparr regulates myoblast cell cycle withdrawal through the activation of miR‐133b, miR‐206, and H19 expression.

Figure 7

  • A
    qRT–PCR for pri‐miR‐133b, pri‐miR‐206, and H19 expression in differentiating C2C12 cells transfected with indicated siRNAs. n = 3, mean ± SD. *P < 0.05. **P < 0.01.
  • B
    ChIP‐qPCR detection of Pol II occupancy at the indicated promoters in Myoparr‐depleted C2C12 cells. The data were normalized to input values. n = 3, mean ± SD. *P < 0.05. Myoparr KD decreased Pol II occupancy at the miR‐133b promoter with a marginal trend toward significance (P = 0.052).
  • C, D
    ChIP‐qPCR detection of Ddx17 (C) and PCAF (D) occupancies at the indicated promoters in Myoparr‐depleted C2C12 cells. n = 3, mean ± SD. *P < 0.05. **P < 0.01.
  • E
    qRT–PCR showing decreased expression of miR‐133b and miR‐206 in both Myoparr‐ and Ddx17‐depleted C2C12 cells. n = 3, mean ± SD. *P < 0.05. **P < 0.01.
  • F
    Western blots showing increased ERK1/2 activity (pERK1/2) and Cdc6 expression in differentiating C2C12 cells 48 h after Myoparr and Ddx17 KD. Tubulin expression served as an internal control.
  • G
    Increased Pola1 expression detected by qRT–PCR. n = 3, mean ± SD. **P < 0.01.
Data information: Statistical analyses were performed using unpaired two‐tailed Student's t‐test. In cases of unequal variances, unpaired two‐tailed Welch's t‐test was used.