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. 2019 Jan 8;20(3):e47468. doi: 10.15252/embr.201847468

Figure 8. Knockdown of Myoparr blocks skeletal muscle atrophy caused by denervation in mice.

Figure 8

  1. Seven days after denervation, weights of innervated (−) and denervated (+) tibialis anterior (TA) muscles were measured. n = 4, mean ± SEM. *P < 0.05 (unpaired two‐tailed Student's t‐test).
  2. Expression of myogenin in innervated and denervated TA muscles detected by qRT–PCR 7 days after denervation. n = 4, mean ± SD. ***P < 0.001 (unpaired two‐tailed Welch's t‐test).
  3. qRT–PCR showing increased Myoparr expression in denervated TA muscles 7 days after denervation. n = 3, mean ± SD. ***P < 0.001 (unpaired two‐tailed Student's t‐test).
  4. Immunoblot showing decreased expression of myogenin by Myoparr depletion in denervated TA muscles 7 days after denervation. Expression of tubulin served as an internal control.
  5. Weights of innervated and denervated TA muscles electroporated either with control or Myoparr shRNA. Muscle weights were measured 7 days after denervation. n = 5 for each group, mean ± SEM. ***P < 0.001. n.s., not significant (unpaired two‐tailed Student's t‐test).
  6. Representative immunostaining images for laminin (red) and EmGFP (green) of innervated or denervated TA muscles electroporated with control or Myoparr shRNA, both containing EmGFP. Scale bar, 100 μm.
  7. Cross‐sectional area (CSA) of electroporated EmGFP‐positive myofibers (250 fibers per sample) was analyzed 7 days after denervation. n = 5 for each group, mean ± SEM. **P < 0.01. n.s., not significant (unpaired two‐tailed Student's t‐test).