FIG 3.

Expression of ATG5, ATG12, and ATG16 is regulated by HuR. Hep3B cells were transfected with siHuR or siCtrl for 48 h, and expression levels of ATG5, ATG12, and ATG16 were assessed by RT-qPCR and Western blotting. (A) The levels of ATG5, ATG12, ATG16, and HuR mRNAs were measured by RT-qPCR. The GAPDH mRNA level was used for normalization. (B) Endogenous ATG5, ATG12, ATG16, LC3, and HuR expression levels were analyzed by Western blotting, and the relative intensities of WB images are shown in the graph. β-Actin was used as a loading control. S.E., short exposure; L.E., long exposure. The data are representative of the results from three independent experiments, and values are expressed as means and SD (Student's t test). *, P < 0.05; **, P < 0.01.