FIG 6.

Correlation between ATG5, ATG12, ATG16, and HuR expression in human liver cell lines and liver tissues. (A) Whole-cell lysates were prepared from human hepatic cell lines (L-02 and THLE3) and human HCC cell lines (Hep3B and Huh7), and endogenous ATG5, ATG12, ATG16, and HuR expression levels in the whole-cell lysates were detected by Western blotting. The intensities of WB images from three independent experiments were analyzed and are shown in the graph. *, P < 0.05; **, P < 0.01. (B and C) ATG5, ATG12, ATG16, and HuR expression levels in liver tissues from HCC patitents were assessed by IHC. (B) Representative images of immunohistochemical detection of ATG5, ATG12, ATG16, and HuR in a human HCC TMA slide. Scale bars = 200 μm. (C) Correlation of HuR expression with ATG5, ATG12, or ATG16 presented in a scatter graph using the IHC stain score of each core (n = 50). HCC, primary HCC; Meta, metastatic HCC; Normal, paired normal tissues. The data represent P and R² values of IHC analysis (37). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant. 0 < R² < 0.09, no correlation; 0.09 < R² < 0.25, low correlation; 0.25 < R² < 0.49, moderate correlation; 0.49 < R² < 0.81, high correlation; 0.81 < R² < 1.0, very high correlation.