FIG 6.

Effect of the miR-155 sponge on tumor growth in nude mice. (a) Representative images showing tumor formation in nude mice, with a graph showing the changes in tumor volume (n = 3). (b) Changes in the weight of tumor xenografts produced by SAS–pLCE–miR-155 sponge and SAS-pLCE cells (n = 3). (c) Relative expression of miR-155 in tumors as determined by qPCR (n = 3 as experimental triplicates). (d) Western blot for Pdcd4, with vinculin acting as internal control and GFP acting as an indicator of effective inhibition of miR-155 in xenografts. T1, T2, and T3 represent xenografts from mice injected with SAS-pLCE cells, whereas T4, T5 and T6 represent xenografts from mice injected with SAS–pLCE–miR-155 sponge cells. Values are expressed as the means ± SD (*, P < 0.05; **, P < 0.01; ***, P < 0.001).