Table 1. Effects of specific inhibitors on the protease activity.
| c(inhibitor)/mM | Residual activity/% | |
|---|---|---|
| 0.1 | 1 | |
| None | 100 | 100 |
| DFP | 10 | 5 |
| Pefabloc | 18 | 9 |
| PMSF | 40 | 25 |
| EDTA | 99 | 95 |
| EGTA | 90 | 90 |
| 1,10-Phenanthroline | 100 | 95 |
| Iodoacetamide | 100 | 100 |
| E-64 | 100 | 100 |
| Pepstatin A | 100 | 100 |
| 10–3 | 10–2 | |
| Bestatin | 100 | 100 |
| TLCK | 100 | 100 |
| 10–4 | 10–3 | |
| Aprotinin | 100 | 100 |
DFP=diisopropyl fluorophosphates, PMSF=phenylmethylsulfonyl fluoride, EDTA=ethylenediaminetetraacetic acid, EGTA=ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium, TLCK= Nα-tosyl--l-lysine chloromethyl ketone hydrochloride