Table 3. Flow cytometry analysis of normal human skin fibroblasts (BJ), colorectal metastatic adenocarcinoma (SW620) and breast epithelial adenocarcinoma (MCF-7) treated with fir (Abies alba Mill.) honeydew honey samples after 24 and 48 h of treatment.
| Cell line/honey sample | t/h | γ/(mg/mL) | N(cell)/% | |||
|---|---|---|---|---|---|---|
| sub-G1 | G1 | S | G2/M | |||
| BJ/2 | 24 | Control | 20.1±1.8 | 49.1±0.6 | 33.9±1.9 | 17.2±2.6 |
| 4.44 | 17.8±1.2 | 52.5±2.7 | 35.9±4.1 | 11.7±1.3 | ||
| 22.20 | (49.4±5.6)* | (63.8±0.8)* | (22.9±0.2)* | (13.4±0.9)* | ||
| 48 | Control | 19.1±0.4 | 83.5±1.3 | 5.7±0.2 | 10.8±1.4 | |
| 4.44 | 19.5±2.6 | 82.4±0.7 | (10.1±0.7)* | 7.7±1.3 | ||
| 22.20 | (71.9±7.6)* | (57.8±0.5)* | (30.8±0.6)* | 11.5±1.1 | ||
| SW620/3 | 24 | Control | 18.0±1.0 | 26.9±3.4 | 56.6±3.2 | 16.6±0.3 |
| 7.96 | 14.9±0.2 | 31.9±2.1 | 47.7±8.2 | 20.5±6.2 | ||
| 39.80 | (24.3±4.8)* | (43.5±1.4)* | (33.2±5.8)* | 23.3±4.4 | ||
| 48 | Control | 27.8±0.1 | 32.2±2.1 | 47.1±0.1 | 20.7±2.3 | |
| 7.96 | 27.5±0.7 | 39.4±4.4 | 46.8±1.8 | 13.8±2.7 | ||
| 39.80 | (46.3±4.6)* | (46.1±3.9)* | 37.6±7.1 | 16.4±3.2 | ||
| MCF-7/4 | 24 | Control | 39.6±3.0 | 41.6±1.6 | 36.7±3.4 | 21.7±1.8 |
| 9.94 | 50.6±5.0 | 35.8±1.7 | 41.6±1.1 | 22.6±0.6 | ||
| 49.70 | (63.6±7.0)* | (29.4±0.8)* | 34.3±1.6 | (36.4±2.5)* | ||
| 48 | Control | 46.1±3.2 | 45.9±3.0 | 18.0±1.4 | 36.2±4.5 | |
| 9.94 | 49.4±5.8 | 45.1±1.9 | 16.8±2.4 | 38.2±0.5 | ||
| 49.70 | (75.9±4.0)* | 36.3±3.3 | (33.1±4.9)* | 30.6±8.1 | ||
Honey samples were selected according to the most potent IC50 value and 5·IC50 specific cell line. Values are expressed as mean±standard deviation. *statistically significant at p<0.05