Skip to main content
. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: J Am Chem Soc. 2018 Oct 24;140(44):14761–14769. doi: 10.1021/jacs.8b08152

Table 2.

Comparison of SPR Binding Kinetics Results for Selected Compoundsa

Compounds ka(M−1 s−1) kd(s−1) KD (nM)
DB2457 (7a) 2.0 ± 0.2 × 106 0.0081 ± 0.001 4.1 ± 0.4
DB2708 (7c) 3.5 ± 0.3 × 106 0.0150 ± 0.002 4.3 ± 0.3
DB2711 (7d) 4.1 ± 0.3 × 106 0.0516 ± 0.004 12.6 ± 0.1
DB2715 (7f) 4.6 ± 0.4 × 106 0.0444 ± 0.004 9.6 ± 0.4
DB2728 (7g) 2.9 ± 0.1 ×106 0.0217 ± 0.001 7.4 ± 0.2
DB2764 (7h) 1.8 ± 0.2 × 106 0.0198 ± 0.002 11.0 ± 0.2
DB2714 (7j) 3.4 ± 0.2 × 106 0.0290 ± 0.002 8.5 ± 0.1
DB2740 (7m) 2.3 ± 0.3 × 106 0.0078 ± 0.001 3.4 ± 0.3
DB2753 (7q) 7.3 ± 0.2 × 104 0.0076 ± 0.001 104 ± 2
DB2712 (7t) 2.6 ± 0.3×106 0.0180 ± 0.002 7.0 ± 0.2
a.

Kinetics analysis was performed by global fitting with a 1:1 binding model. ka is the association rate constant, while kd is the dissociation rate constant. KD was calculated through global fitting of the kinetic data obtained for various concentrations of the compounds; KD is given by kd / ka. The kinetics errors are based on two independent experiments and the data fitting of different concentrations of compounds. The data of DB2457, DB2711, DB2714 and DB2740 are based on three independent experiments.