Table 2.

Details of the loci and primers used in the molecular study

Locus1	Primer	Primer sequence (5′–3′)	Orientation	Amplicon size	Reference
SSU-ITS-LSU	V9G	TTAAGTCCCTGCCCTTTGTA	Forward		de Hoog and Gerrits van den Ende (1998)
	LR5	TCCTGAGGGAAACTTCG	Reverse	ca 1.6 kb	Vilgalys and Hester (1990)
	ITS42	TCCTCCGCTTATTGATATGC	Reverse		White et al. (1990)
	LR32	CCGTGTTTCAAGACGGG	Reverse		Vilgalys and Hester (1990)
	LR2R-A2	CAGAGACCGATAGCGCAC	Forward		Voglmayr et al. (2012)
cal	CAL-228F	GAGTTCAAGGAGGCCTTCTCCC	Forward		Carbone and Kohn (1999)
	CAL-737R	CATCTTTCTGGCCATCATGG	Reverse	458 bp	Carbone and Kohn (1999)
his	CYLH3F	AGGTCCACTGGTGGCAAG	Forward		(Crous et al. (2004)
	H3-1b	GCGGGCGAGCTGGATGTCCTT	Reverse	438 bp	Glass and Donaldson (1995)
ms204	MSE1F1n1	AAGGGNACYCTSGAGGGCCAC	Forward		Voglmayr and Mehrabi (2018)
	MS-E5R2n	CCASAGCATGGTGGTRCCRTC	Reverse	ca 1 kb	Voglmayr and Mehrabi (2018)
rpb1	RPB1-Af	GARTGYCCDGGDCAYTTYGG	Forward		Stiller and Hall (1997)
	RPB1-6R1asc	ATGACCCATCATRGAYTCCTTRTG	Reverse	ca 1.2 kb	Hofstetter et al. (2007)
rpb2	fRPB2-5F	GAYGAYMGWGATCAYTTYGG	Forward		Liu et al. (1999)
	fRPB2-7cR	GGGGWGAYCAGAAGAAGGC	Reverse	ca 1.2 kb	Liu et al. (1999)
	dRPB2-5f	GAYACNGAYGAYCGWGAYCAYTTYGG	Forward		Voglmayr et al. (2016)
	dRPB2-7r	AANCCCATDGCYTGYTTDCCCAT	Reverse	ca 1.2 kb	Voglmayr et al. (2016)
tef1	EF1-728F	CATCGAGAAGTTCGAGAAGG	Forward		Carbone and Kohn (1999)
	TEF1-LLErev	AACTTGCAGGCAATGTGG	Reverse	ca 1.3 kb	Jaklitsch et al. (2005)
	TEF1_INTF2	CCGTGAYTTCATCAAGAACATG	Forward		Jaklitsch (2009)
	TEFD_iR2	GTCTGGCCATCCTTGGAGAT	Reverse		Voglmayr et al. (2018)
tub2	T1	AACATGCGTGAGATTGTAAGT	Forward		O’Donnell and Cigelnik (1997)
	BtHV2r	CATCATRCGRTCNGGGAACTC	Reverse	ca 1 kb	Voglmayr et al. (2017)
	T1D	CAANATGCGTGAGATTGTRAGT	Forward		This study
	T22D	CATCATRCGRTCNGGGAACTC	Reverse	ca 1.6 kb	This study

¹SSU-ITS-LSU, partial nuclear 18S rDNA, internal transcribed spacers and intervening 5.8S rDNA and 28S rDNA amplified and sequenced as a single fragment; cal, calmodulin; his, histone H3; ms204, guanine nucleotide-binding protein subunit beta; rpb1, DNA-directed RNA polymerase II largest subunit; rpb2, DNA-directed RNA polymerase II second largest subunit; tef1, translation elongation factor 1-alpha; tub2, β-tubulin

²Internal sequencing primers