Table 2.
Analytical performance of the 7-plex immunoassay
| Mean (pg/mL) | Intra-assay Precision (%CV) | Inter-assay Precision (%CV) | LOB (pg/mL) | LLOQ (pg/mL) | ULOQ (pg/mL) | Triplicates* (%CV) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| QC1 | QC2 | QC1 | QC2 | QC1 | QC2 | |||||
| OPN | 13650.0 | 1435.0 | 4.4 | 6.8 | 5.2 | 14.5 | 278.8 | 389.5 | 40024.5 | 7.1 |
| MIA | 670.0 | 67.5 | 2.3 | 3.9 | 7.0 | 14.2 | 12.5 | 16.3 | 1999.0 | 4.7 |
| CEACAM-1 | 9540.0 | 967.5 | 7.5 | 7.4 | 5.4 | 20.8 | 351.2 | 548.5 | 30079.5 | 5.0 |
| MIC-1 | 2977.5 | 297.5 | 3.0 | 3.6 | 3.5 | 5.0 | 3.0 | 68.5 | 7407.0 | 3.5 |
| SPON1 | 7825.0 | 762.5 | 2.9 | 2.9 | 2.8 | 4.5 | 50.8 | 185.3 | 24145.0 | 3.2 |
| POSTN | 8100.0 | 710.0 | 2.9 | 4.0 | 3.4 | 10.2 | 146.1 | 186.3 | 25081.3 | 3.4 |
| HSP27 | 637.5 | 62.5 | 3.0 | 2.5 | 3.2 | 8.0 | 5.3 | 16.0 | 2001.3 | 2.7 |
QC1, high control. QC2, low control. LOB, limit of black. LLOQ, lower limit of quantitation. ULOQ, upper limit of quantification. *mean of %CV for triplicates in all samples for each protein