Fig. 6.

Binding of EBNA1 to cellular mRNAs in cellulo; (a) RIP-qPCR validation of RNA binding targets of the EBNA1 protein. RIP experiments from biological triplicates of HEK293T and HEK293T-EBNA1 cells were carried out, and immunoprecipitated RNAs were quantified in qPCR and normalized to their respective input RNA. HIST1H2BJ, HIST1H4H, RPL10A and RPS3AP6 were statistically enriched in EBNA1 immunoprecipitated fraction compared to control immunoprecipitation. Unpaired t-test, * p < 0.05, ** p < 0.005; (b) AS-PCR on potential AS site detected in the RIP-Seq experiment. Primers were designed to amplify ASEs potentially bound by EBNA1. Control and EBNA1 cells were compared to detect any change in AS in these regions. No significant change could be detected for any potential AS regions bound by EBNA1