Fig. 7.

Autophagy induction regulates the migration and invasion of CRC cells. a-c In FAT4-overexpressing HCT116 and LOVO cells, the expression levels of P62 and Twist1 were increased after treatment with the autophagy inhibitor MHY1485, but these levels were reduced in FAT4-knockdown SW480 cells treated with the autophagy activator Torin 1. In addition, the LC3-II/LC3-I expression ratio was decreased in FAT4-overexpressing HCT116 and LOVO cells treated with MHY1485 and increased in FAT4-knockdown SW480 cells treated with Torin 1. d Transwell assays revealed that cell migration and invasion were promoted after autophagy was blocked in FAT4-overexpressing HCT116 and LOVO cells by treatment with the autophagy inhibitor MHY1485. In FAT4-knockdown SW480 cells, the promotion of autophagy by treatment with the autophagy activator Torin 1 inhibited cell migration and invasion. *P < 0.05, as determined by Student’s t-test