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Sensitivity of PCR for the P. gingivalis-specific 16S rRNA gene. PCR was performed with a set of primers and genomic DNA from P. gingivalis ATCC 33277 Lane M: 100-bp ladder; lane N: without DNA; lanes 1 to 5: 0.1, 1, 10, 100, and 1,000 pg DNA, respectively; lanes 6 to 9; 10 ng of DNA specimens obtained from periodontally healthy subjects.
