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Measurement hMSC coverage. hMSC cultured on (A) aligned and (B) flat PDMS substrates with different surface coatings under hypoxic (2% O2) environment. Cytoskeleton and cell nuclei were observed by F-actin (red) and DAPI (blue) staining, respectively. Quantification of percentage area covered by hMSCs on (C) day 3, (D) day 6 and (E) day 9 using ImageJ software. PDMS/PD and PDMS/PD/collagen samples showed significantly increased hMSC coverage compared to other groups.
