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. 2019 Feb 25;25:1480–1488. doi: 10.12659/MSM.914799

Figure 2.

Figure 2

Impacts of PM on the expression of autophagy-associated proteins and mRNA were determined by western blotting and RT-qPCR in HK-2 cells. β-actin was used as an internal control. Expression of (A, B) Beclin-1, (C, D) LC-3II, and (E, F) P62 was determined by western blotting and RT-qPCR. NG – normal glucose, 5.5 mM glucose; HG – high glucose, 30 mM glucose; NG+PM – PM (1 mM) plus 5.5 mM glucose; HG+PM – PM (1 mM) plus 30 mM glucose. (# P<0.05, ## P<0.01 compared with NG. * P<0.05, ** P<0.01 compared with the HG group). PM – pyridoxamine; RT-qPCR – reverse transcription-quantitative polymerase chain reaction; HK-2 – human proximal tubular epithelial cells.