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. 2019 Feb 25;25:1480–1488. doi: 10.12659/MSM.914799

Figure 4.

Figure 4

PM inhibits ROS generation in HG-induced HK-2 cells. DCHF-DA fluorescence intensity was analyzed quantificationally by flow cytometry. Data were expressed as means ± standard deviation. NG – normal glucose, 5.5 mM glucose; HG – high glucose, 30 mM glucose; NG+PM – PM (1 mM) plus 5.5 mM glucose; HG+PM – PM (1 mM) plus 30 mM glucose; (# P<0.05, ## P<0.01 compared with NG. * P<0.05, ** P<0.01 compared with the HG group). PM – pyridoxamine; ROS – reactive oxygen species; HK-2 – human proximal tubular epithelial cells; DCHF-DA – dichlorodihydrofluorescein diacetate.