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. 2018 Nov 27;28(6):992–1006. doi: 10.1093/hmg/ddy411

Figure 6.

Figure 6

ER stress levels do not increase in S63del/HANI/+ mice. (A) WB analysis of P28 sciatic nerves lysates shows activation of the ER stress markers Bip/Grp78, Grp94 and P-eIF2-alpha in S63del mice as compared with WT. No further increase was detected in the expression levels of these markers between S63del and HANI/+//S63del mice. Tubulin was used as loading control. One representative experiment of three is shown. (B) Densitometric quantification of Bip, Grp94, calnexin and p-eIF2-alpha relative protein levels. (C) qRT-PCR for BiP, CHOP and spliced XBP-1 mRNA in P28 sciatic nerves. All the stress makers are increased in S63del as compared with WT, but we detected no differences in their levels of expression between S63del and HANI/+//S63del nerves. Each experiment was repeated three times on different pools of three nerves per genotype. *P < 0.05, **P-value < 0.01 and ***P < 0.001 by one-way ANOVA with Bonferroni’s multiple comparison test.