Figure 7.

The expression of negative regulators of myelination is not altered by Nrg1TIII overexpression. qRT-PCR and WB analysis (with relative densitometric quantification) on P28 nerves for Sox2 (A) and Id2 (B). Both factors are increased in S63del nerves as compared with WT, but remained similarly increased in HANI/+//S63del mice. Each qRT-PCR experiment was repeated three times on different pools of three nerves per genotype. For WB, one representative experiment of three is shown. Tubulin was used as loading control, equal loading is also shown by coomassie staining; ns, not significant, by one-way ANOVA with Bonferroni’s multiple comparison test.