Table 1.
Strains and plasmids used in this study.
| Strains or plasmids | Description | References |
|---|---|---|
| E. coli strains | ||
| BL21(DE3) | Used as host strain | Invitrogen |
| BL21-22AB | E. coli BL21(DE3) harboring plasmid pET22b-DavAB | This study |
| BL21-YDT | E. coli BL21(DE3) harboring plasmid pACYC-GabDT | This study |
| BL21-22AB-YDT | E. coli BL21(DE3) harboring plasmid pET22b-DavAB and pACYC-GabDT | This study |
| Plasmids | ||
| pET22b | expression vector, AmpR, PT7, pBR322 ori | This study |
| pACYCDuet-1 | expression vector, CmR, PT7,P15A ori | This study |
| pACYC-GabDT | Gene GabD inserted between NcoI and HindIII sites of pACYCDuet-1 and GabT inserted between NdeI and XhoI sites of pACYCDuet-1 | This study |
| pET22b-DavAB | Gene DavA inserted between AgeI and XmaI sites of pET22b and DavB inserted between NdeI and XhoI sites of pET22b | This study |