Fig.2.

Characterization of NP103 compound as GSK-3 selective inhibitor. A, B) NP103 inhibits GSK-3β and GSK-3α in vitro. Data represent the mean values + SD of the percentage of enzymatic activity. Each data point was assayed in duplicates. IC50 values were calculated from the concentration-response curves. C) Effect of different ATP concentrations on GSK-3β activity. A double-reciprocal Lineweaver-Burk plotting of the enzyme kinetics in the presence of two different concentrations of NP103 is shown. Each point represents the mean value of two independent experiments. No competition with substrate was observed (not shown). D) NP103 kinase inhibition selectivity profile. A comparison among external and in-house obtained results in selected kinases.