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. 2019 Feb 28;12:1705–1716. doi: 10.2147/OTT.S196506

Figure 1.

Figure 1

Expression of CD24 in human HCC clinical specimens and cell lines.

Notes: (A) Amino acid sequence alignment of CD24A with CD24B; (B) qPCR was carried out to evaluate the mRNA expression of CD24A in 90 pairs of HCC tissues and corresponding adjacent non-cancerous liver tissues; (C) Western blotting was performed to detect the expression of CD24 in 4 pairs of HCC tissues (K) and corresponding adjacent non-cancerous liver tissues (N); (D) mRNA expression levels of CD24A and CD24B in human HCC cell lines were detected by qPCR; (E) the protein expression level of CD24 in human HCC cell lines was determined by Western blotting; (F) the mRNA expression levels of CD24A and CD24B in SK-Hep1 and Li7 cells stably transfected with CD24A, CD24B or control (pWPXL) vectors; (G) Western blot analysis of CD24 protein in SK-Hep1 and Li7 cells stably transfected with CD24A, CD24B or control (pWPXL) vectors. **P<0.01.