Table 3.
Candidate receptors expressed in carp macrophages.
| Description | Accession number | CTLD | ITAM | TM | RPKM | CRD | MG | cypCar |
|---|---|---|---|---|---|---|---|---|
| zgc:174904 | NP_001170922.1 | 1 | 0 | 1 | 836 | X | 00031274 | |
| Asialoglycoprotein receptor 1-like isoform 1 | XP_026096377.1 | 2 | 1 | 1 | 654 | X | X | 00032252 |
| Mannose receptor c type 1 | ALS87701.1 | 2 | 2 (6) | 0 | 467 (354) | 00046879, (00034006) | ||
| Asialoglycoprotein receptor 1-like isoform X2 | XP_026098956.1 | 1 | 0 | 1 | 239 | X | X | 00032253 |
| Ladderlectin-like | XP_026129733.1 | 1 | 0 | 0 | 215 | 00046054 | ||
| C-type lectin domain family 4 member E-like | XP_026087308.1 | 1 | 0 | 1 | 200 | 00044344 | ||
| C-type lectin domain family 4 member C | NP_001313501.1 | 1 | 1 | 1 | 165 | X | 00024225 | |
| Macrophage mannose receptor 1-like | XP_026142830.1 | 2 (5) | 1 (7) | 1 | 92 (60) | X | 00047225, (00023855) | |
| CD209 antigen | XP_003197805.3 | 1 | 0 | 0 | 83 | 00049168 | ||
| C-type lectin domain family 4 member E | XP_002660626.3 | 1 | 0 | 0 | 60 | 00014657 |
Candidates were selected based on a minimal expression of ≥ 50 RPKM in unstimulated carp macrophages or differential expression following stimulation of macrophages with β-glucans. Description refers to the closest BLASTx hit, with associated NCBI protein accession code. Number of conserved CTL domains (CTLDs), ITAM sequences and transmembrane domains (TM) in the protein sequence are included. RPKM refers to the average (n = 5) reads per kilobase million in unstimulated macrophages and is a measure for gene expression. CRD and MG refer to differentially expressed genes in macrophages after stimulation with curdlan (CRD) or MacroGard® (MG) based on RNAseq analysis (highlighted in bold). cypCar codes identify common carp genes (Cyprinus carpio). Names or numbers in brackets refer to genes with identical BLASTx hits (duplicated genes). Candidate proteins are ordered based on their expression (RPKM).