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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Biomaterials. 2018 Nov 8;192:560–568. doi: 10.1016/j.biomaterials.2018.10.040

Figure 4. Pore characterization of B-GelMA.

Figure 4.

(a) 3D confocal projection of B-GelMA scaffolds. Void space is imaged by incubating scaffolds in FITC-labeled dextran. (b) Process overview for pore size analysis. 2D slices were analyzed using a custom-built MATLAB algorithm to detect void spaces between the annealed beads. Void area was converted to circles of equal area to extrapolate equivalent diameter. (c) Void space fraction for B-GelMA scaffolds, prepared using varying crosslinking times, i.e., varying matrix stiffness. (d) Median pore diameter of B-GelMA scaffolds versus crosslinking time. The porosity and void fraction of B-GelMA scaffolds are independent of the matrix stiffness. Accordingly, B-GelMA generates a protein-based microporous scaffold with orthogonal porosity and stiffness.