Table 1. List of parameters required for ZooNavigator .
The values are typical conditions for multiple small-wedge data collection by ZOO.
| Parameter name | Example for ‘small-wedge’ scheme |
|---|---|
| Mode | Small wedge |
| Puck ID | CPS1974 |
| Pin ID | 1–16 |
| Total oscillation per wedge (°) | 5.0 |
| Oscillation width (°) | 0.1 |
| Exposure time for raster scan (s) | 0.02† |
| Photons per frame in 2D raster scan | 1010 † |
| Beam size (µm) | 10 (horizontal) × 15 (vertical)† |
| Dose per wedge (MGy) | 10.0 |
| Exposure time for data collection (s per frame) | 0.05 |
| Camera distance (mm) | 200 |
| Loop size (µm) | 600 |
| No. of spots for good crystals | 15–100‡ |
| Data directory | /data/here/ |
| Data name | multi |
| Maximum No. of data collections per loop | 100 |
†
Typical value for finding crystals for challenging samples, such as GPCRs.
‡
Lower and upper thresholds for SHIKA can be set. If the number of spots exceeds 100, the grids are neglected in this case.