Table 1. Antibodies used for immunohistochemistry.
| Antigen | Conjugate | Species | Company | Catalog # | Lot/Batch # | Antibody Registry # | Clonality | Dilution |
|---|---|---|---|---|---|---|---|---|
| Primary Antibodies | ||||||||
| Keratin 20 | None | Guinea Pig | Abcam | ab192682 | GR314080-5 | NA | Polyclonal | 1:1000 |
| Keratin 18 | None | Rabbit | Abcam | ab24561 | GR92020-1 | AB_1310117 | Polyclonal | 1:250 |
| Keratin 19* | None | Mouse | ThermoFisher | MA1-91210 | QD2006615 | AB_1955705 | Monoclonal | 1:400 |
| Human Leukocyte Antigen (HLA)-ABC | FITC | Mouse | Accurate Chemical | CLHLA01F | 0131B | AB_10060657 | Monoclonal | 1:50 |
| Keratin 15 | None | Chicken | Bio Legend | PCK-153P | D14BF00272 | AB_291540 | Polyclonal | 1:250 |
| Collagen IV | None | Rabbit | Yo Proteins | 507 | 60607 | AB_2082817 | Polyclonal | 1:50 |
| E-Cadherin | None | Rabbit | Novus | NB110-56937 | NA | AB_843761 | Monoclonal | 1:200 |
| Synaptophysin** | None | Rabbit | Abcam | ab32127 | GR312544-10 | AB_2286949 | Monoclonal | 1:400 |
| Neurofilament Heavy | None | Chicken | Neuromics | CH22104 | 402107 | AB_1619710 | UNK | 1:7500 |
| Neurofilament Medium | None | Chicken | Abcam | ab39371 | GR259030-4 | AB_726980 | Polyclonal | 1:1000 |
| Secondary Antibodies: | ||||||||
| Chicken IgG (H+L) | AF-594 | Goat | Molecular Probes | A11042 | 1504513 | AB_142803 | UNK | 1:400 |
| Guinea Pig IgG (H+L) | AF-594 | Goat | Novus | NBP1-75746 | 1841755 | AB_11005097 | UNK | 1:400 |
| Guinea Pig IgG (H+L) | AF-488 | Goat | Molecular Probes | A11073 | 26-54-062312 | AB_142018 | UNK | 1:400 |
| Rabbit IgG (H+L) | AF-594 | Donkey | Molecular Probes | A21207 | 1938375 | AB_141637 | UNK | 1:400 |
| Mouse IgG (H+L) | AF-594 | Donkey | Molecular Probes | A21203 | 987237 | AB_141633 | UNK | 1:400 |
Temp, temperature; FITC, fluorescein isothiocyanate; AF, Alexa Flour; NA, not available; UNK, unknown; RT, room temperature.
*For this antibody, the Mouse on Mouse Detection Kit (Vector Laboratories) was used for detection; see text for details.
**For this antibody, the Tyramide SuperBoost Kit (ThermoFisher) was used for signal amplification; see text for details.