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. 2019 Feb 21;15(2):e1007977. doi: 10.1371/journal.pgen.1007977

Fig 5. Cell proliferation is significantly increased from endothelial lineage loss of VGLL4 valves.

Fig 5

(A) Schematic view of recombined Vgll4 allele and experimental strategy were used to create valves composed of VGLL4 semi-knockout (RFP-) and VGLL4 total knockout (RFP+) VIC populations. (B) Immunoflorecence analyses of RFP positive proliferating cells on P10 heart arterial valves from Tie2Cre+;Vgll4fl/-;RosafsRFP/+ (n = 5) and Tie2Cre+;Vgll4fl/+;RosafsRFP/+ controls (n = 5). DAPI is in blue, EdU is in green, VE-cad is in gray. (C) Quantification of the percentage from RFP-,EdU+ and RFP+,EdU+ cells per aortic or pulmonary valve. (D) Immunoflorecence analyses of RFP positive proliferating cells on P10 heart arterial valves from WntCre+;Vgll4fl/-;RosafsRFP/+ (n = 5) and WntCre+;Vgll4fl/+;RosafsRFP/+ (n = 5) controls. DAPI is in blue, EdU is in green, VE-cad is in gray. (E) Quantification of the percentage from RFP-,EdU+ and RFP+,EdU+ cells per aortic or pulmonary valve. AoV: aortic valve; PV: pulmonary valve. White arrows point proliferating endothelial lineage cells. Scale Bar = 100μm.***P<0.005, ns: no significance.