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. 2019 Feb 21;15(2):e1007607. doi: 10.1371/journal.ppat.1007607

Fig 4. RPS27A and C2 protein regulate JAZ1 degradation to manipulate plant defense.

Fig 4

(A) Expression of RPS27A gene in RPS27A-silencing tobacco plants. Values are means±SE, n = 8. (B) Survival rate of whitefly on control and rps27A-silenced tobacco. Values are means±SE, n = 30. (C) Daily number of eggs laid by per female whitefly on control empty-vector-inoculated and rps27A-silenced tobacco plants. Values are means±SE, n = 30. (D) Relative expression of RPS27A gene in RPS27A overexpressing (OE-RPS27A) plants. Values are means±SE, n = 8. (E) Survival rate of adult whitefly on control and RPS27A overexpressing (OE-RPS27A) tobacco plants. Values are means±SE, n = 30. (F) Daily number of eggs laid by per female whitefly on control and RPS27A overexpressing (OE-RPS27A) plants. Values are means±SE, n = 30. (G) Expression of terpene-related genes [epi-arisotolchene synthase (EAS), epi-arisotolchene synthase 12 (EAS12), terpenoid cyclase (Cyclase)] in control and RPS27A-silencing tobacco plants. Values are means±SE, n = 8. (H) Expression levels of MYC2 in control and RPS27A-silencing tobacco plants. Values are means±SE, n = 8. (I) Effects of whitefly infestation on content of RPS27A protein. (J) & (K) RPS27A-silencing and C2 overexpression affect the degradation of JAZ1 protein. Accumulation of JAZ1 protein in control, whiteflies infested, rps27A-silencing or C2 expressing plants was detected by western blot. Same amount of leave samples were collected and JAZ1 protein content was detected by western blot. Asterisks indicate significant differences between different treatments (P < 0.05; Student’s t test for all experiments). All experiments were repeated twice with similar results.