Table 1. Binding affinities of SETD3 proteins to β-actin peptides.
| β-actin peptide | SETD3 (aa 2–502) |
Stoichiometric coefficient (N) |
Kd (µM) |
|---|---|---|---|
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | Wildtype | 1.0 | 0.17 ± 0.04 |
| 66TLKYPIEAGIVTNWDDMEKIWHH88 (H73A) | Wildtype | 1.3 | 0.45 ± 0.08 |
| 1ARTKQTARKSTGGKAPRKQLATK23 (H3K4) | Wildtype | *N/A | *NB |
| 25ARKSAPATGGVKKPHRYRPGTVA47 (H3K36) | Wildtype | N/A | NB |
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | R215A | 1.2 | 3.6 ± 1.0 |
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | N256A | 0.94 | 2.1 ± 0.4 |
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | N256D | 1.3 | 23 ± 7 |
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | N256Q | 1.1 | 2.5 ± 0.5 |
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | W274A | 0.94 | 0.51 ± 0.10 |
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | Y313F | 0.86 | 3.0 ± 0.1 |
| 66TLKYPIEHGIVTNWDDMEKIWHH88 | R316A | 1.1 | 7.4 ± 0.9 |
| 66TLKAPIEHGIVTNWDDMEKIWHH88 (Y69A) | Wildtype | 1.4 | 3.8 ± 0.8 |
| 66TLKYPAEHGIVTNWDDMEKIWHH88 (I71A) | Wildtype | 1.3 | 16 ± 3 |
| 66TLKYPIEHGIVTNWADMEKIWHH88 (D80A) | Wildtype | 1.3 | 3.5 ± 0.4 |
| 66TLKYPIEHGIVTNWDAMEKIWHH88 (D81A) | Wildtype | 1.3 | 2.0 ± 0.5 |
| 66TLKYPIEHGIVTNWDDAEKIWHH88 (M82A) | Wildtype | 1.2 | 0.89 ± 0.08 |
| 66TLKYPIEHGIVTNWD80 | Wildtype | 1.0 | 2.9 ± 0.7 |
*N/A: Not applicable. *NB: no binding affinity detectable by ITC.
Dissociation constants (Kds) were derived from a minimum of two experiments (mean ± S.D.). The original binding curves are shown in Supplementary file 1.