Table 2. Data collection and refinement statistics.
| SETD3–β-actin | SETD3-methylated β-actin | |
|---|---|---|
| Protein Data Bank ID | 6ICV | 6ICT |
| Data collection | ||
| Radiation wavelength (Å) | 0.9789 | 0.9792 |
| Space group | P 1 21 1 | P 1 21 1 |
| Cell dimensions | ||
| a, b, c (Å) | 60.19, 175.17, 66.50 | 59.98, 176.69, 125.89 |
| α, β, γ (°) | 90, 92.57, 90 | 90, 93.37, 90 |
| Resolution (Å) | 35.00–2.15 (2.23–2.15) | 72.27–1.95 (2.06–1.95) |
| Rmerge | 0.135 (0.609) | 0.169 (0.868) |
| I / σI | 14 (3.4) | 8.1 (2.6) |
| CC1/2 | 0.988 (0.884) | 0.991 (0.827) |
| Completeness (%) | 100(100) | 99.1 (99.7) |
| Redundancy | 6.4 (6.4) | 7.0 (7.0) |
| Refinement | ||
| Resolution (Å) | 35.00–2.15 | 59.87–1.95 |
| No. of reflections (used/free) | 71998/3629 | 187163/9084 |
| Rwork/Rfree | 0.168/0.205 | 0.178/0.214 |
| Number of atoms/B-factor (Å2) | 8493/30.7 | 16956/33.7 |
| Protein | 7574/30.3 | 15147/33.1 |
| Peptide | 276/32.9 | 591/38.4 |
| AdoHcy | 52/19.3 | 104/20.0 |
| Solvent | 591/36.2 | 1114/40.0 |
| RMSD bonds (Å)/angles (°) | 0.007/0.84 | 0.007/0.87 |
| Ramachandran Plot favored/allowed/outliers (%) | 98.76/1.24/0 | 98.98/1.02/0 |
Values in parentheses are for the highest-resolution shell.