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. 2019 Mar 4;151(3):279–281. doi: 10.1085/jgp.201812315

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© 2019 Robertson

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Figure 1. — The problem of conformational ambiguity of BetP distance data. The inward-facing (4C7R, yellow) and outward-facing (4LLH, red) conformations of the Na⁺/betaine cotransporter BetP show small structural changes of 2–3 Å RMSD. Distances of C_α atoms between G450 and S516 are 28.7 ± 0.1 Å and 29.7 ± 0.8 Å respectively, calculated and averaged over the three subunits in the crystal structures. Structural alignment, RMSD, and distance calculations were performed using the analysis program VMD (Humphrey et al., 1996).