Table 5. Constants derived from kinetic parameters.
| Constanta | Source | Definition |
|---|---|---|
| [S]0.5 | VmaxH(app) | Half saturation of the maximal rate in co-ion–dependent rate measurements (Eq. 31) |
| [H]0.5 | VmaxS(app) | Half saturation of the maximal rate in substrate-dependent rate measurements (Fig. 2) |
| KDS(app)high | KDS(app) | Limit of apparent affinity in the high–co-ion concentration domain (Fig. 3) |
| KDS(app)low | KDS(app) | Limit of apparent affinity in the low–co-ion concentration domain (Fig. 3) |
| [H]y = 0 | KDS(app) | Intercept of linear parts of apparent affinities in the low– and high–co-ion concentration domains (Online supplemental material, Figure S4.2) |
| nhigh | KDS(app) | Number of co-ions from slope linear part in the high–co-ion concentration domain (Fig. 3) |
| nlow | KDS(app) | Number of co-ions from slope linear part in the low–co-ion concentration domain (Fig. 3) |
| nN-t | VmaxS(app) | Hill coefficient of maximal rate in substrate-dependent rate measurements |
| nmax | n(app) | Highest Hill coefficient of co-ion–dependent rate measurements (Fig. 5) |
a
t is the number of co-ions binding before the substrate (Online supplementary material file S3).