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. 2019 Mar 4;151(3):292–315. doi: 10.1085/jgp.201812213

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© 2019 Tilley et al.

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Figure 5. — Kv2.1 open probability is decreased by GxTx in single-channel recordings. (A) Voltage protocol used to classify single-channel traces. Currents recorded with 1 µM GxTx in the pipette solution were classified as “free” or “bound” based on the presence or lack of openings at 0 mV, respectively. (B) Single-channel current responses to protocol shown in A after subtraction of leak currents and capacitive transients. On-cell patches. Gray, “control”; blue, “free”; red, “bound.” (C) Fraction of trials classified as “bound” in the absence (gray) or presence (red) of 1 µM GxTx. Circles, individual patches. Horizontal bars, arithmetic means. (D) Average single-channel currents during the +75-mV segment. Dotted lines, zero current level. Smooth lines, fits of Eq. 3 (control: A = 0.97 ± 0.23 pA, τ_act = 5.2 ± 2.6 ms, σ = 1.05 ± 0.21; free: A = 1.06 ± 0.16 pA, τ_act = 6.0 ± 2.2 ms, σ = 1.39 ± 0.29; bound: A = 0.60 ± 0.09 pA, τ_act = 15.5 ± 8.8 ms, σ = 2.10 ± 0.82). (E) τ_act from average single-channel currents as in D. Circles, individual patches; gray lines connect measurements from the same patch. Horizontal lines, geometric means. (F) Mean single-channel current between 100 and 500 ms after stimulation to +75 mV. Horizontal lines, arithmetic means. (G) Current histograms at +75 mV from patches in B. Dotted lines, single-channel current half amplitude from Gaussian fits to histogram peaks. (H) Mean single-channel current amplitude from Gaussian histogram fits to individual patches. Horizontal lines, arithmetic means. (I) Open probability determined from integral of current histograms above half-amplitude threshold. Horizontal lines, arithmetic means.