Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 4;151(3):292–315. doi: 10.1085/jgp.201812213

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Tilley et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — GxTx stabilizes long-lived closed states of Kv2.1. (A) Gray, blue, and red lines: idealized single-channel traces during the +75 mV segment of the voltage protocol in Fig. 5 A. Gray, control; blue, “free” with 1 µM GxTx; red, “bound” with 1 µM GxTx. (B) Cumulative probability of latencies to the first opening. Smooth lines, fits of Eq. 3 (control A = 0.934 ± 0.038 pA, τ_act = 5.14 ± 0.39 ms, σ = 1.95 ± 0.61; free A = 0.98 ± 0.03 pA, τ_act = 7.0 ± 2.5 ms, σ = 1.45 ± 0.24; bound A = 0.934 ± 0.006 pA, τ_act = 29.5 ± 1.3 ms, σ = 1.40 ± 0.29). (C) τ_act from first latencies to first opening as in B. Circles, individual patches; gray lines connect measurements from the same patch. Horizontal lines, geometric means. (D) Open probability after the first opening derived from idealized traces. Horizontal lines, arithmetic means. (E) Log-binned open dwell-time distributions and single exponential fits. y axis is square root scaling. Dashed lines indicate microscopic time constant of closing. (F) Microscopic time constant of closing from individual patches. Horizontal lines, geometric means. (G) Log-binned closed dwell-time distribution and multi-exponential fit from representative patches. Two exponential components for control and free traces, three exponential components for bound traces. Dashed lines indicate time constant of channel opening. (H) Time constants of channel opening from individual patches. Horizontal lines, geometric means. (I) Probability (prob.) of channel-closing events that are longer than 50 ms. Horizontal lines, arithmetic means.