Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 4;151(3):342–356. doi: 10.1085/jgp.201812263

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Kapoor et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Effect of amitriptyline on gA activity in the single-channel assay. (A) Single-channel current traces recorded with increasing concentrations of amitriptyline added to both sides of a DC_18:1PC/n-decane bilayer doped with gA⁻(13) and AgA(15). The lines denote the current transition amplitudes for gA⁻(13) (red) and AgA(15) (blue). (B) Current transition amplitude histograms of gA⁻(13) and AgA(15). The darker the shading, the greater the amitriptyline concentration. In the absence of the drug, the characteristic current transition peaks were 3.2 ± 0.1 pA and 2.0 ± 0.1 pA for AgA(15) and gA⁻(13), respectively. 100 µM amitriptyline shifted the two peaks to 2.8 ± 0.1 and 1.8 ± 0.1 pA. (C and D) Normalized single-channel survivor histograms (black, solid lines) of AgA(15) (C) and gA⁻(13) (D). The histograms were fit with single exponential distributions (Eq. 7; red dotted lines), with lifetimes (τ).