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. 2019 Mar 4;216(3):539–555. doi: 10.1084/jem.20182184

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© 2019 Croft et al.

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Figure 2. — Multiple CNS cell types can be targeted simultaneously in BSCs or cotransduced using rAAV vectors. (A) Organotypic BSCs were prepared and transduced at 0 DIV with three rAAVs (1–2 × 10¹⁰ VGs per well): a MAP-2 promoter to express YFP packaged in rAAV2/8 (green), a hCBA promoter to express human WT tau packaged in rAAV2/8 (red), and a GFAP promoter to express EBFP packaged in rAAV2/8 (blue), fixed at 28 DIV. Then fluorescence throughout the entire BSC was imaged. Bar, 200 µm. (B) Organotypic BSCs were prepared and transduced at 0 DIV with two rAAVs: a hCBA promoter to express EGFP packaged in rAAV2/8 (green), and a hCBA promoter to express mApple (red), fixed at 14 DIV. Then fluorescence throughout the entire BSC was imaged to identify colocalization. Bar, 50 µm.