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. 2019 Mar 4;216(3):704–720. doi: 10.1084/jem.20180823

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© 2019 Michels et al.

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Figure 6. — Specific protein signatures for normal and oncogenic Wnt signaling. (A) Venn diagrams show limited overlap of proteins up- or down-regulated after Wnt-receptor (extrinsic) stimulation and APC loss-of-function (intrinsic). Proteins were filtered for log twofold changes ± 1 and P < 0.25. (B) Global correlation shows independence of intrinsic and extrinsic responses. (C) Unsupervised clustering of up-regulated proteins marks distinct Wnt-receptor and APC-KO signatures. (D) Ingenuity pathway analysis. Significantly enriched gene ontology terms for Wnt-receptor and APC-KO protein signature are shown (red and blue bars) that were further grouped into biological categories. (E) WB validation of proteins. Lysates from normal and APC-KO organoids (n = 3 isogenic pairs) cultured in the presence of Wnt/R-spondin were probed. ACTIN was used for normalization. See also Fig. S3 and Table S1, C and D.