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. 2019 Mar 4;218(3):1039–1054. doi: 10.1083/jcb.201807175

Figure 2.

Figure 2.

Localization of factors involved in cytoskeletal organization in migrating AV ECs. (A–H) Immunohistochemistry of 58-hpf Tg(nfatc1:GAL4); Tg(UAS:EGFP-CAAX) (A–F) or Tg(nfatc1:GAL4); Tg(UAS:EGFP) (G and H) embryos. Green, GFP; cyan, DAPI; magenta (A–H) and white (A′–H′), specific factors of interest. (A–F) Localization of FA-associated factors. (A and B) Itgβ1 (A) and Itgα5 (B) appeared enriched in discrete puncta at the migrating cell protrusions. (C–E) Cytoplasmic FA adaptor molecules Talin1 (C), Vinculin (D), and p-Pax (E) appeared strongly localized in the leading edge of migrating cells. (F) The Itgα5β1 ligand Fn appeared distributed around the endocardial and myocardial cells at the AVC. (G and H) The tight junction–associated protein ZO-1 and adherens junction protein VE-cadherin were localized between the leading and follower AV ECs but appeared undetectable in the EC protrusions. Dashed red lines outline the leading edge of the migrating cells at a distance of 1–2 µm. Scale bars: (A–F) 20 µm; (A′–F′ and G–H′) 10 µm.