Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 4;218(3):771–782. doi: 10.1083/jcb.201807102

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Sallé et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Magnetic cortical pulling domains drive marked asymmetric divisions. (A) Magnetic beads injection, cap formation, and induced asymmetric division. (B–E) Time-lapse images of the cleavage of sea urchin zygotes. (B) The cap is formed with beads with strong MT minus end activity and maintained; (C) a noninjected control embryo; (D) the pulling cap is not maintained; and (E) a control using a cap formed with beads that do not interact with MTs. Yellow stars: female/zygote nucleus; red stars: blastomere nuclei after division. (F) Divided blastomere area ratio in conditions B to E (n = 10, n = 8, n = 11, and n = 10 cells). (G) Quantification of the angle (Δα) between the division axis and the axis from the cell center to the cap (n = 12, n = 17, and n = 10 cells). (H) Blastomere area ratio plotted as a function of the angle (β) formed by the edges of the pulling cap and the cell center. The dashed line is a visual guide. Results were compared by using a two-tailed Mann–Whitney test. ns, P > 0.05; ****, P < 0.0001. Error bars are SD. Bars, 20 µm.