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. 2019 Mar 4;218(3):771–782. doi: 10.1083/jcb.201807102

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© 2019 Sallé et al.

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Figure 3. — Cortical pulling forces remain constant during and after aster centration. (A) The method used to measure cortical pulling forces. (B) Time-lapse of aster centration, and concomitant reduction of the magnetic force holding the cap. Top: Low-magnification view of the embryo moving away from the probe (left). Bottom: Details of cap detachment dynamics. Yellow stars: the aster center; arrowheads: the cap. (C) Typical example of the evolution of the distance between the cell cortex and the cap (gray), and of the magnetic force (red), as a function of the distance between the egg and magnet. Arrowhead: the cap “take-off.” (D) Average distance between cap and cortex plotted as a function of the magnetic force, during centration (in green; n = 16) and after centration (in red; n = 18). Dashed lines are SD. Take-off forces (arrows) correspond to cortical forces exerted by domains onto asters and were determined using the fits (continuous lines; see Materials and methods). (E) Cortical forces plotted as a function of cap volumes. Red dashed line: linear regression. Pearson r and P value are displayed on the graph. Bars, 20 µm.