Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 4;218(3):961–976. doi: 10.1083/jcb.201807154

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Locke and Thorner

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — GTP-bound Vps21 stimulates TORC2 phosphorylation of Ypk1. Equivalent samples of cultures of WT (BY4741) or yMLT42 (vps21Δ ypt52Δ ypt53) cells expressing Ypk1^7A-myc from its native promoter on a CEN plasmid (pFR252), and carrying either empty vector (−; pRS426) or coexpressing FLAG-Vps21 (pMLT101), FLAG-Vps21^Q66L (pMLT103), or FLAG-Vps21^S21L (pMLT102) from the GAL promoter on the same vector, were grown to mid-exponential phase in 2% raffinose-0.2% sucrose medium, shifted for 1 h to galactose medium (2% final concentration), then treated for 2 h with ethanol (–) or an equivalent volume of the same solvent containing AbA (1.8 µM final concentration; +), harvested, lysed, resolved by Phos-tag SDS-PAGE, and analyzed by immunoblotting (IB).