| BG1805 |
2 µm, URA3, GAL1prom, C-terminal tandem affinity (TAP) tag vector |
Open Biosystems |
| pMAL-c5X |
Bacterial expression vector for production of MBP (MalE) fusion proteins |
New England BioLabs |
| pESC-Leu |
2 µm, GAL1/10prom, LEU2
|
Agilent |
| YCpLG |
CEN, LEU2, GAL1/10prom vector |
Bardwell et al., 1998 |
| pRS425 |
2 µm, LEU2 vector |
Sikorski and Hieter, 1989 |
| pRS426 |
2 µm, URA3 vector |
Sikorski and Hieter, 1989 |
| pRS315 |
CEN, LEU2 vector |
Sikorski and Hieter, 1989 |
| pET30a |
Bacterial expression vector |
Novagen |
| pMLT35 |
pMAL-c5X MBP-MUK1-(His)6
|
This study |
| pMLT74 |
pMAL-c5X MBP-muk1(S171A S172A S173A S183A S184A S185A)-(His)6
|
This study |
| pMLT22 |
pESC-LEU GAL1prom-MUK1-Myc |
This study |
| pMLT56 |
pESC-LEU GAL1prom-muk1(1-220)-Myc |
This study |
| pMLT57 |
pESC-LEU GAL1prom-muk1(1-220; S171A S172A S173A S183A S184A S185A)-Myc |
This study |
| pAX50 |
BG1805 YPK1(L424A)-TAP |
Muir et al., 2014 |
| pFR252 |
pRS315 ypk1(S51A T57A S71A T504A S644A S653A T662A)-Myc |
Muir et al., 2015 |
| pAM20 |
pRS315 Ypk1-myc |
Roelants et al., 2011 |
| pMLT101 |
pRS426 GAL1prom-FLAG-VPS21
|
This study |
| pMLT102 |
pRS426 GAL1prom-FLAG-vps21(S21L) |
This study |
| pMLT103 |
pRS426 GAL1prom-FLAG-vps21(Q66L) |
This study |
| pMLT110 |
pRS426 GAL1prom-FLAG-YPT7
|
This study |
| pMLT114 |
pRS426 GAL1prom-FLAG-mNG |
This study |
| pMLT111 |
pRS426 MSB3-FLAG |
This study |
| pMLT83 |
pRS315 MUK1-3xFLAG |
This study |
| pMLT84 |
pRS315 muk1(S171A S172A S173A S183A S184A S185A)-3xFLAG |
This study |
| pMLT85 |
pRS315 muk1(D353A)-3xFLAG |
This study |
| pMLT92 |
pRS315 MUK1(S171E S172E S173E S183E S184E S185E)-3xFLAG |
This study |
| pMLT132 |
pET30a ypk1(D242 D470A)-(His)6
|
This study |
| pMLT135 |
pMAL-c5X MBP-Ypk1(603-680) [MBP-Ypk1CT] |
This study |
| pMLT138 |
pMAL-c5X MBP-Ypk1(603-680; S644 S653A T662A S671A S672A S678) [MBP-Ypk1CT (6A)] |
This study |
| pMLT88 |
pET30a VPS21-(His)6
|
This study |
| pMLT141 |
pET30a YPT7-(His)6
|
This study |
| pPL495 |
pRS315 PHSlm1-YPK1-3xHA |
Niles et al., 2012 |
| pMLT115 |
pRS425 GAL1prom-GST |
This study |
| pML116 |
pRS425 GAL1prom-GST-Ypk1 |
This study |
