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. 2019 Mar 4;218(3):1027–1038. doi: 10.1083/jcb.201806148

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© 2019 Heymann et al.

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Figure 4. — Expression and secretion from HEK-293 cells of WT and mutant retinoschisin. (A) Reducing SDS-PAGE of the cells. The spike 1 mutants (G91V, W92S, and Y93A) are better retained in the cells than the WT and spike 3 mutant (R141H). (B) Reducing SDS-PAGE of the medium. Compared with WT and R141H, W92S and Y93A are poorly secreted, and G91V is secreted not at all. (C) Nonreducing SDS-PAGE of the medium. All secreted RS1 form disulfide cross-linked octamers. (D) Blue-native gel of the medium. All secreted RS1 form double octamers.