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. 2019 Mar 4;218(3):993–1010. doi: 10.1083/jcb.201805099

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© 2019 Patrón et al.

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Figure 8. — DCAF12 controls synaptic GluRIIA levels in a Cul4-dependent manner. (A–C) Effects of postsynaptic DCAF12 OE and reduced Cul4 on synaptic GluRIIA levels (A and B; n ≥ 11) and mEPP amplitudes at larval NMJs (C; n ≥ 9). Scale bar, 5 µm. (D and E) Effects on resting potential (D) and input resistance (E) of muscle (n ≥ 9). (F) Effect of muscle Cul4 KD on synaptic GluRIIA levels (n ≥ 13). (G–I) mEPP amplitudes (G), EPP amplitudes (H), and quantal content (I) of heterozygous glurIIA-cul4 double mutants (n ≥ 9). Graphs display means ± SEM. Statistical analysis used one-way ANOVA (B–E and G–I) or two-tailed unpaired t test (F); *, P < 0.05; **, P < 0.01; ***, P < 0.001.