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. 2019 Mar 4;218(3):820–838. doi: 10.1083/jcb.201811038

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© 2019 Delaney et al.

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Figure 5. — Transcripts up-regulated in absence of lin-65 correlate with and are largely epistatic to met-2-induced changes. (A) Representative image of embryos bearing reporter gwIs4, which expresses GFP::LacI when derepressed, following RNAi against met-2, lin-65, or empty vector. Bar = 5 µm. (B) RT-qPCR on total RNA isolated from wild-type, lin-65(gw1465), met-2(n4256), and arle-14(tm6748) mutant embryos for selected repetitive elements showing the fold change in expression over wild-type (N = 4; error bars indicate the standard deviation). Classes of repetitive elements are indicated above the graph. (C and D) Changes of genes in met-2(n4256), lin-65(gw1465), and met-2;lin-65 embryos determined by total RNA-seq (N = 3). (C) Correlation between the fold change (FC, log2) over wild-type for gene expression between met-2 and lin-65 single and double mutants. Genes significantly changed compared with wild-type (FDR <0.05; fold change >2 or <−2) are colored according to the genotype (lin-65 = cyan; met-2 = red; met-2 = purple), and genes significantly changed in two compared genotypes are black. Pearson correlations are indicated as R² in the graphs. (D) Expression level of genes in reads per kilobase per million (RPKM in log2) in met-2 and lin-65 single mutants (y axis) compared with the lin-65;met-2 double mutant (x axis). Black mark genes significantly changed in both met-2 and lin-65 single mutants.