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. 2019 Mar 4;218(3):820–838. doi: 10.1083/jcb.201811038

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© 2019 Delaney et al.

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Figure 6. — MET-2 and LIN-65 are essential to recover from temperature stress. (A) Representative images of MET-2::FLAG::mCherry, GFP::PCN-1, and LIN-65::FLAG::GFP embryos subjected to 37°C heat shock for the indicated length of time (N = 3, n = 60 embryos). (B and C) Quantitation of MET-2::FLAG::mCherry (B) and GFP::PCN-1 (C) foci/nucleus from A; blue bars indicate mean foci number. (D) Mean survival of animals 24 h after embryonic heat shock. N = 2, n (wt 0 min) = 520, n (wt 30 min) = 463, n (wt 60 min) = 436, n (met-2 0 min) = 213, n (lin-65 0 min) = 238, n (lin-65 30 min) = 239, n (lin-65 60 min) = 243, n (met-2 30 min) = 229, and n (met-2 60 min) = 210; error bars indicate standard deviation.